Copywriter Pat Friesen recommends testing special characters in subject lines. She often uses vertical bars (a.k.a., pipes) instead of commas to | visually | separate | elements | in subject lines.
[Brackets], {braces}, and +s also link, separate, and/or save character space and draw the eye. Example: [Free 42-Page Ebook] The Smart Guide to Lead Generation. She says not to put a period at the end of your subject line.
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Source: Target Marketing, 6/28/13